Nucleic Acid Extraction Or Purification Kit
Nucleic Acid Extraction Or Purification Kit or stored at -20℃. The sample should be are transported using 0℃ curling.
Introduction
The Nucleic Acid Extraction Or Purification Kit (Magnetic Beads Method) is designed for the automated purification of RNA and DNA from body fluids (such as swabs, plasma, serum) using automated nucleic acid extraction instruments. Magnetic-particle technology provides high-quality DNA/RNA that is suitable for direct use in downstream applications such as amplification or other enzymatic reactions.
Application Range
The whole blood, plasma, serum and other tissue samples were directly lysed and digested. The released nucleic acid was selectively adsorbed by super paramagnetic nanometer magnetic beads. Then the protein, inorganic salt ions and organic impurities were removed by washing solution.Finally, the nucleic acid was eluted with eluent to obtain a pure nucleic acid solution.
Kit Contents
| Cat. No. | YXN-VIRAL01-32A-BR | Components | ||
| -50A | - 100A | |||
| Size | 32Tes | 50Test | 100Test | |
| Lysis Buffer | 96 wellPre-pack
ed Plates 2 piecces |
25ml | 50ml | Surfactant and Tris |
| Wash Buffer I | ★15ml | ★30ml | High-salt solution | |
| Wash Buffer Il | ★6ml*2 | ★12ml*2 | Low-salt solution | |
| Elution Buffer | 10ml | 20ml | Low-salt solution | |
| MagaBio Reagent | 1.0ml | 2.0ml | Magnetic particles | |
| Handbook(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Notes:For YXN-VIRAL01-32A-BR-50A, add 15mL Absolute ethanol to ★15mL Wash Buffer I before use; add 24mL Absolute ethanol to ★6mL Wash buffer Il before use. | ||||
| For YXN-VIRAL01-32A-BR-100A, add 30mL Absolute ethanol to ★30mL Wash Buffer I before use; add 48mL Absolute ethanol to ★12mL Wash buffer Il before use.【Reagents to be prepared by the user】Please prepare the absolute ethanol (analytical grade) by yourself. | ||||
Storage Conditions
Upon arrival of the kit, kit components can be stored at room temperature (15 − 25°C). The reagents are stable for up to one year from the manufacturing date.
Sample Requirements
1. Applicable sample: Swabs, plasma, serum and whole blood etc.
2. Sample storage and transportation: The sample should be tested immediately
Materials and Devices Required but Not Provided
1. Disposable powder-free gloves
2. Biohazard container
3. Pencil or per
Procedure
The following uses sample strip extraction swab lotion as an example to briefly explain the operation steps of the extraction reagent on the biological nucleic acid extraction instrument Bioer NPA-32P or SMART 32. For other sample types, please refer to the user manual. It can also be operated by customers according experimental acquirement:
1. Reagent Preparation
a. For YXN-VB03-32A-50A and YXN-VB03-32A-100A
Add 500uL Lysis Buffer to the column 1 and 7 of the 2.2mL 96-deep-well plate, 500uL Wash Buffer I to the column 2 and 8, 500uL Wash Buffer II to the column 3, 4 and 9,10; 70uL Elution Buffer to the column 5 and 11, 180uL pure water and 20uL MagaBio Reagent to the column 6 and 12 (the magnetic beads should be mixed thoroughly before use),
b. For YXN-VB03-32A
Put the 96 well pre-packed reagents at room temperature. Shake 96-well plate upside down for three times, and tear off the plastic bag. Centrifuge the pre-packed reagent for a few seconds (or swing by hand a few times) to avoid reagent adhering to the wall of the tubes. Tear off the aluminum foil film of 96-well plate and identify the direction of the plate (magnetic beads in column #6 and #12),
2.Sample Extraction
1. Add 300uL sample to the 96 well plate columns #1 and #7, please avoid cross-contamination,
2. Place 96 deep well plate to the instrument, install the 8-strip tips on the instrument,
3. Run the program according to the following procedures,
4. After the automatic purification is over, transfer the elution Buffer in columns 5 and 11 to a clean antinuclear 0.5mL centrifuge tube; if not using it immediately, please store at-20 °C.
Performance Characteristics
1. The extracted product is detected by high-sensitivity HBV DNA detection reagent to reach a sensitivity of 10 IU/mL. The extracted product is detected by high-sensitivity HCV RNA detection reagent to reach a sensitivity of 50 IU/mL.
2. Select 4 samples (serum/plasma sample, nasopharyngeal swab sample, cervical exfoliated cell sample), each sample is diluted 10 times with 3 gradients(including the original sample total of 4 concentrations), using qualified reagents and tests agents to detect internal reference gene according to the product instructions, and the Ct value of each batch differs by less than 1.
| Step | Well Location | Program Name | Waiting Time(min:SS) | Mixing Time(min:SS) | Magnet Time(min:SS) | Adsorption | Speed | Volume tatus(μL) | Temperature |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Beads | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wash1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wash2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wash3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Discard | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. GENERAL SAFETY.
Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document.
1.1 Before using an instrument or device, read and understand the safety information provided in the user documentation provided by the manufacturer of the instrument or device.
1.2 Before handling chemicals, read and understand all applicable Safety Data Sheets (SDSs) and use appropriate personal protective equipment (gloves, gowns, eye protection, etc.). To obtain SDSs, see the “Documentation and Support” section in this document.
2. Chemical safety
GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below, and consult the relevant SDS for specific precautions and instructions: Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials..
2.1 Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing).
2.2 Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood).
2.3 Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer’s cleanup procedures as recommended in the SDS.
2.4 Handle chemical wastes in a fume hood.
2.5 Ensure use of primary and secondary waste containers. (A primary waste
container holds the immediate waste. A secondary container contains spills or leaks from the primary container. Both containers must be compatible with the waste material and meet federal, state, and local requirements for container storage.).
2.6 After emptying a waste container, seal it with the cap provided.
2.7 Characterize (by analysis if necessary) the waste generated by the particular applications, reagents, and substrates used in your laboratory.
2.8 Ensure that the waste is stored, transferred, transported, and disposed of according to all local, state/provincial, and/or national regulations.
2.9 Radioactive or biohazardous materials may require special handling, and disposal limitations may apply.
3.Biological hazard safety
Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards.
BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations. Wear appropriate protective equipment, which includes but is not limited to: protective eyewear, face shield, clothing/lab coat, and gloves. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially infectious materials.
Read and follow the applicable guidelines and/or regulatory requirements in the following:
In the U.S.: U.S. Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories found at: www.cdc.gov/biosafety.
Occupational Safety and Health Standards, Bloodborne Pathogens (29 CFR§1910.1030), found at:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Your company’s/institution’s Biosafety Program protocols for working with/handling potentially infectious materials. Additional information about biohazard guidelines is available at: www.cdc.gov.
In the EU: Check local guidelines and legislation on biohazard and biosafety precaution and refer to the best practices published in the World Health Organization (WHO) Laboratory Biosafety Manual, third edition, found at: www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
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